Phosphatase activity tracer
Zymography is a powerful techniques to quantify phosphatase activity along the root using quantitative imaging. This page provides an imageJ plugin that extract the profiles of phosphatase activity along the root. The plugin is initiated with a coarse outline of the root centerline. An algorithm is then used to refine the position of the centerline along the root. The profile of activity is then extracted perpendicular to the centerline and exported as a CSV file. Python scripts are also provided to post-proces the data. scripts consists of an optimisation algorithm to find the enter of the root more accurately but also the fitting of models to extract key features of the profile.
This file is the ImageJ plugin (.java)
This file is the Python script to proces data extracted from images (.py)